Absolutely not. These rumors purported by overzealous rivals have never been substantiated with any evidence. In addition, the City of Auburn expressed mourning over his passing by placing black ribbons on trees and flying flags at halfstaff.
The first college football game played in the United States was on November 6, 1869 between Rutgers and Princeton with Rutgers winning 6-4. Click on the 'First College Game' link on this page to read a detailed history of the game.